Fibrifold a fibroblast with Cure CMD and the reticular fibers!

In mathematics, a fibrifold is (roughly) a fiber space whose fibers and base spaces are orbifolds. A wallpaper group (or plane symmetry group or plane crystallographic group) is a mathematical classification of a two-dimensional repetitive pattern, based on the symmetries in the pattern. In mathematics, a distributive lattice is a lattice in which the operations of join and meet distribute over each other.

A fibroblast is a type of biological cell that synthesizes the extracellular matrix and collagen, produces the structural framework (stroma) for animal tissues, and plays a critical role in wound healing. Fibroblasts are the most common cells of connective tissue in animals.

Reticular fibers, reticular fibres or reticulin is a type of fiber in connective tissue composed of type III collagen secreted by reticular cells. Reticular fibers crosslink to form a fine meshwork (reticulin). This network acts as a supporting mesh in soft tissues such as liver, bone marrow, and the tissues and organs of the lymphatic system.

Magnesium is a cofactor in more than 300 enzyme systems that regulate diverse biochemical reactions in the body, including protein synthesis, muscle and nerve function, blood glucose control, and blood pressure regulation. Magnesium is required for energy production, oxidative phosphorylation, and glycolysis. I was researching magnesium batteries in my last SBIR ESCO article and am interested in biochemical reactions in the body.

Magnetic space group, Graphene reinforced CSA Cement, minimum-cost flow problem (MCFP)

In physics, magnetic skyrmions (occasionally described as 'vortices,' or 'vortex-like' configurations) are quasiparticles which have been predicted theoretically and observed experimentally in condensed matter systems.

Magnesium diboride is the inorganic compound with the formula MgB₂. It is a dark gray, water-insoluble solid. The compound has attracted attention because it becomes superconducting at T_c = 39K (-389.47 Fahrenheit)

Room Temperature Superconductors and Energy Sean McLaughlin, December 17, 2016

Paramagnetic compounds (and atoms) are attracted to magnetic fields while diamagnetic compounds (and atoms) are repelled from magnetic fields.

In quantum mechanics, the Planck time (

t P

) is the unit of time in the system of natural units known as Planck units. A Planck time unit is the time required for light to travel a distance of 1 Planck length in a vacuum, which is a time interval of approximately 5.39 × 10 ⁻⁴⁴ s

For paramagnetic Planck time sake!

Diamagnetic materials are repelled by a magnetic field; an applied magnetic field creates an induced magnetic field in them in the opposite direction, causing a repulsive force. In contrast, paramagnetic and ferromagnetic materials are attracted by a magnetic field.

A Most Unusual Superconductor and How It Works First-principles calculation explains the strange behavior of magnesium diboride

Paul Preuss, August 4, 2002

Most metals aren't magnetic with the exception of iron, nickel, cobalt, gadolinium, neodymium and samarium. Because Nickel (Ni) is ferromagnetic it is used in making Alnico magnets (consisting of aluminium, nickel, and cobalt). Magnetic alloys have become common, especially in the form of steel (iron and carbon), alnico (iron, nickel, cobalt, and aluminum), and permalloy (iron and nickel).

Ask a mathematician, ask a physicist:

Q: What causes iron, nickel, and cobalt to be attracted to magnets, but not other metals?
Two types of electrons: bound electrons and free electrons. The free electrons are free to move between atoms, and are the cause of conductivity in metals. The bound electrons are stuck to the individual atoms.

“Lenz’s law” or “the universe is a stubborn jerk law”

Boron is used for building strong bones, treating osteoarthritis, as an aid for building muscles and increasing testosterone levels, and for improving thinking skills and muscle coordination.

Potassium is an electrolyte with many essential jobs: It helps conduct nerve impulses and muscle contractions, regulates the flow of fluids and nutrients into and out of body cells, and helps keep your blood pressure in check—partly by countering the effects of sodium.

A potassium-ion battery or K-ion battery (abbreviated as KIB) is a type of battery and analogue to lithium-ion batteries, using potassium ions for charge transfer instead of lithium ions. It was invented by the Iranian/American chemist Ali Eftekhari (President of the American Nano Society) in 2004

Silver Calcium alloy batteries are a type of lead-acid battery with grids made from lead-calcium-silver alloy, instead of the traditional lead-antimon.

Biological Significance of Iron, Zinc, Copper, Molybdenum, Cobalt, Chromium, Vanadium, and Nickel Concentrations of transition metals and zinc in sea water and human plasma.

The concentration of calcium within muscle cells is controlled by the sarcoplasmic reticulum, a unique form of endoplasmic reticulum in the sarcoplasm. Muscle contraction ends when calcium ions are pumped back into the sarcoplasmic reticulum, allowing the muscle cell to relax.

In this SBIR ESCO research article this Memorial day 2019, I want to honor my friend Carole Zoom and my family who practice medical arts as well teach.

I am working on a SBIR research article for proposing with Cure CMD

Cure CMD was founded in 2008 by three parents whose children were affected by Congenital Muscular Dystrophy. Through close collaboration with scientists, doctors, dedicated volunteers, involved families, and generous donors, Cure CMD has achieved significant impact in its first decade as a nonprofit organization

Collagen 6 (Ullrich-Bethlem)

Tendon Extracellular Matrix Alterations in Ullrich Congenital Muscular Dystrophy

Original Research ARTICLE

Front. Aging Neurosci., 08 June 2016

Nutrition for Healthy Connective Tissue

Proteoglycans are proteins that are heavily glycosylated. The basic proteoglycan unit consists of a "core protein" with one or more covalently attached glycosaminoglycan (GAG) chain(s). The point of attachment is a serine (Ser) residue to which the glycosaminoglycan is joined through a tetrasaccharide bridge (e.g. chondroitin sulfate-GlcA-Gal-Gal-Xyl-PROTEIN).

Connective Tissues: Bone, Adipose, and Blood

In biology, adipose tissue, body fat, or simply fat is a loose connective tissue composed mostly of adipocytes. In addition to adipocytes, adipose tissue contains the stromal vascular fraction (SVF) of cells including preadipocytes, fibroblasts, vascular endothelial cells and a variety of immune cells such as adipose tissue macrophages.

ColVI myopathies:

where do we stand, where do we go?

Published: 23 September 2011

CONNECTIVE TISSUE

Galvani also invented galvanized iron and the galvanometer. Luigi Galvani (in Bologna) disagreed with theories posed by Alessandro Volta (in Pavia, about 150 miles from Bologna). ... Galvani believed this happened because the human body works with animal electricity, in fluid form, flowing from the brain through the nerves into the muscles where electricity produces motion.

Dancing Italian Frogs and Toadies,

Stooges v. Prescription Thugs,

Olympic Gold

By David Hirsch

It is interesting to note Galvani and Volta were both Italian as well Guglielmo Marconi, and Bruno Pontecorvo... for piss and vinegar sake, froggy!

The percolation threshold is a mathematical concept in percolation theory that describes the formation of long-range connectivity in random systems.

Electrical and Rheological Percolation

in Polystyrene/MWCNT

Nanocomposites

Published 2007

Rheology (/riːˈɒlədʒi/; from Greek ῥέω rhéō, "flow" and -λoγία, -logia, "study of") is the study of the flow of matter, primarily in a liquid state, but also as "soft solids" or solids under conditions in which they respond with plastic flow rather than deforming elastically in response to an applied force. It is a branch of physics which deals with the deformation and flow of materials, both solids and liquids.

Glucuronic acid (from Greek γλυκύς "sweet" and οὖρον "urine") is a uronic acid that was first isolated from urine (hence the name). It is found in many gums such as gum arabic (c. 18%) and xanthan, and is important for the metabolism of microorganisms, plants and animals.

What Does Bad Breath

Have to Do with Diabetes?

An odor of ammonia is associated with kidney disease. Well, how do you like that?

Ammonium ions are formed in the breakdown of amino acids

Excretion and the liver

Myoglobin is an iron- and oxygen-binding protein found in the muscle tissue of vertebrates in general and in almost all mammals. It is distantly related to hemoglobin which is the iron- and oxygen-binding protein in blood, specifically in the red blood cells.

Granulocytes are a category of white blood cells characterized by the presence of granules in their cytoplasm. White blood cells (also called leukocytes or leucocytes and abbreviated as WBCs) are the cells of the immune system that are involved in protecting the body against both infectious disease and foreign invaders.

Amino Acid Blood Level

To say that people are “full of piss and vinegar” is to say that they are brimming with energy. Although many speakers assume the phrase must have a negative connotation, this expression is more often used as a compliment, “vinegar” being an old slang term for enthusiastic energy.

Some try to make this expression more polite by substituting “pith” for “piss,” but this change robs it of the imagery of acrid, energetically boiling fluids and conjures up instead a sodden, vinegar-soaked mass of pith. Many people who use the “polite” version are unaware of the original.

The liver is one of the largest organs in the body. It has many important metabolic functions. It converts the nutrients in our diets into substances that the body can use, stores these substances, and supplies cells with them when needed. It also takes up toxic substances and converts them into harmless substances or makes sure they are released from the body.

CellBiology @ Yale

Connective Tissues

Gene therapy creates boy’s replacement skin from his stem cells

Gene therapy may be innovated for other conditions.

Efficient CRISPR/Cas9-mediated editing of trinucleotide repeat expansion in myotonic dystrophy patient-derived iPS and myogenic cells

Nucleic Acids Research, Volume 46, Issue 16, 19 September 2018

Viruses Can Scatter Their Genes Among Cells and Reassemble

Some viruses can replicate without passing all their genes into any one cell.

A classical tenet of virology is that viruses fully infect individual cells and replicate within them. But recent work shows that some viruses instead scatter their genes among many cells. The cells then make new complete viruses by sharing the products of the genes.

By Viviane Callier

CRISPR gene editing relieves muscular dystrophy symptoms in dogs

The technique may one day be used to help people with the muscle-wasting disease

Gene editing of dogs offers hope for treating human muscular dystrophy

Using your brain or using your muscles?

A trion is a localized excitation which consists of three charged quasiparticles. A negative trion consists of two electrons and one hole and a positive trion consists of two holes and one electron. The trion itself is a quasiparticle and is somewhat similar to an exciton, which is a complex of one electron and one hole. The trion has a ground singlet state (spin s = 1/2) and an excited triplet state (s = 3/2).

The Consumer Product Safety Improvement Act (CPSIA)

Mindful muscle memory innovations...

The Center for Consumer Information & Insurance Oversight

Serenity Sells, inside sells and innovations TX HUB SBIR is partnering with Mirlok Studios in a chartered venture, suggesting:

Integral psychology may address emotional agility technology gap(s) in the lattice of developmental lines with wearable (AI) sensors.

Force Sensing Football Helmet

We are making a helmet for football or rather integrating into helmets that exist that allow us as parents to monitor impact remotely.

Additional technologies may be deployed for various sectors.

Gingerbreadman map to rhetorical kissing number charge conjugation with helicity in a neutron magnetic moment

Investigational Therapies

for

Collagen Type VI-Related Disorders

Collagen VI is a protein that plays an essential role in the proper function and health of muscle cells. Two of the genes are located on the long arm of chromosome 21 (21q22.3) and the other is found on the long arm of chromosome 2 (2q37).

I am understanding artificial intelligence, genetic programming (GP )

and clarifying Biological engineering, or bioengineering/bio-engineering, is the application of principles of biology and the tools of engineering to create usable, tangible, economically viable products.

Collagen VI-related myopathy

My name is David Hirsch and I do business as Serenity Sells, inside sells and innovations. I solicit academic teaming agreements to address technology gaps as an agent of change, here from Austin Texas. From a previous SBIR ESCO article, I mentioned:

In computer science and operations research, a genetic algorithm (GA) is a metaheuristic inspired by the process of natural selection that belongs to the larger class of evolutionary algorithms (EA).

PHAT Python API reference

Genetic algorithms

Gray code genetic algorithm

Due to the Hamming distance properties of Gray codes, they are sometimes used in genetic algorithms. They are very useful in this field, since mutations in the code allow for mostly incremental changes, but occasionally a single bit-change can cause a big leap and lead to new properties.

A phonon is a quantized mode of vibration occurring in a rigid crystal lattice, such as the atomic lattice of a solid.

Lattice dynamics

The equations in this section do not use axioms of quantum mechanics but instead use relations for which there exists a direct correspondence in classical mechanics.

For example: a rigid regular, crystalline (not amorphous), lattice is composed of N particles. These particles may be atoms or molecules. N is a large number, say of the order of 10²³, or on the order of Avogadro's number for a typical sample of a solid. Since the lattice is rigid, the atoms must be exerting forces on one another to keep each atom near its equilibrium position. These forces may be Van der Waals forces, covalent bonds, electrostatic attractions, and others, all of which are ultimately due to the electric force.

please refer to my SBIR article for more details in research findings:

Van der Waerden test of maximum clique problem with Waring's conjecture upon Van der Waals force

By David Hirsch

First observation of native ferroelectric metal

Silicene

Indium(III) selenide

Stanene grows on silver

Polar metal

Ferroelectric switching in Indium(III) selenide: potential for ultra-low energy electronics

and

Graphene and van der Waals Materials

please refer to my SBIR article for more details in research findings:

Van der Waerden test of maximum clique problem with Waring's conjecture upon Van der Waals force

By David Hirsch

...Since the lattice is rigid, the atoms must be exerting forces on one another to keep each atom near its equilibrium position. These forces may be Van der Waals forces, covalent bonds, electrostatic attractions, and others, all of which are ultimately due to the electric force.

The potential energy of the entire lattice is the sum of all pairwise potential energies multiplied by a factor of 1/2 to compensate for double counting:

Other lattices include a linear chain, which is a very simple lattice which we will shortly use for modeling phonons. (For other common lattices, see crystal structure.)

The potential energy of the lattice may now be written as

If your going to go the Hamming distance, with or without cheese, might as well consider:

Guglielmo Marconi, 1st Marquis of Marconi (Italian: [ɡuʎˈʎɛlmo marˈkoːni]; 25 April 1874 – 20 July 1937) was an Italian inventor and electrical engineer, known for his pioneering work on long-distance radio transmission, development of Marconi's law, and a radio telegraph system. He is credited as the inventor of radio, and he shared the 1909 Nobel Prize in Physics with Karl Ferdinand Braun "in recognition of their contributions to the development of wireless telegraphy".

The Perennial Philosophy is a comparative study of mysticism by the British writer and novelist Aldous Huxley. Its title derives from the theological tradition of the philosophia perennis.

Gustav Theodor Fechner (/ˈfɛxnər/; German: [ˈfɛçnɐ]; 19 April 1801 – 18 November 1887) was a German philosopher, physicist and experimental psychologist. An early pioneer in experimental psychology and founder of psychophysics, he inspired many 20th-century scientists and philosophers. He is also credited with demonstrating the non-linear relationship between psychological sensation and the physical intensity of a stimulus via the formula:

S=K\ln I

, which became known as the Weber–Fechner law.

A lesson in Heyting algebra for h8trs in the Neighbourhood (mathematics)

By David Hirsch

The noosphere (/ˈnoʊ.əsfɪər/; sometimes noösphere) is the sphere of human thought. The word derives from the Greek νοῦς (nous "mind") and σφαῖρα (sphaira "sphere"), in lexical analogy to "atmosphere" and "biosphere". It was introduced by Pierre Teilhard de Chardin in 1922 in his Cosmogenesis.

For vegetarian's sake researching a cure for muscular dystrophy by shifting metaphor gears of meat heads as if ethos of a bureaucratic, head banging, stone walling Archie Bunker!

To coerce with out consent and Anger Turned Inward

Hotelling's linear city model going the Manhattan distance in the heart of the great state of Texas

CODE SWITCH

This year’s Nobel Prize in economics was awarded to a Python convert

By Dan Kopf

October 8, 2018

Hotelling’s linear city

Advanced Microeconomics:

Slutsky Equation, Roy�s Identity and Shephard's Lemma

I am the Walras's law and the Carpenter's Walrasian auction

Lecture 8: Product Differentiation

with

lectures on quantitative economic modeling

for a

Random walk in a color of law case

Investigational Therapies

Information on current clinical trials is posted on the Internet at www.clinicaltrials.gov. All studies receiving U.S. government funding, and some supported by private industry, are posted on this government web site.

Genetic engineering

Python is becoming the world’s

most popular coding language

But its rivals are unlikely to disappear

Applications for Muscular Dystrophy:

Tissue engineering

The Python programing language may model solutions

Genetic Programing in Python

applying

Gray code genetic algorithm

As to model:

Collagen alpha-2(VI) chain is a protein that in humans is encoded by the COL6A2 gene.

in context of

Variants in the COL6A1, COL6A2, and COL6A3 Genesin Collagen VI-related Congenital Muscular Dystrophy (P2.042)

You can find the HHS SBIR/STTR Omnibus Solicitations, and all of our other SBIR/STTR solicitations on the Funding page of our website: https://sbir.nih.gov/funding.

A signal peptide (sometimes referred to as signal sequence, targeting signal, localization signal, localization sequence, transit peptide, leader sequence or leader peptide) is a short peptide (usually 16-30 amino acids long) present at the N-terminus of the majority of newly synthesized proteins that are destined towards the secretory pathway.

Sharing Secrets: How Bacteria Communicate

Protein targeting or protein sorting is the biological mechanism by which proteins are transported to their appropriate destinations in the cell or outside it.

The piezoresistive effect is a change in the electrical resistivity of a semiconductor or metal when mechanical strain is applied. In contrast to the piezoelectric effect, the piezoresistive effect causes a change only in electrical resistance, not in electric potential.

A Priestley space is an ordered topological space

(X, τ,\leq)

, i.e. a set

X

equipped with a partial order

\leq

and a topology

τ

, satisfying the following two conditions:

$(X, τ)$ is compact.
If ,then there exists a clopen up-set $U$ of $X$ such that $x \in U$ and $y \notin U$ . (This condition is known as the Priestley separation axiom.)

The Golgi apparatus, also called Golgi complex or Golgi body, is a membrane-bound organelle found in eukaryotic cells (cells with clearly defined nuclei) that is made up of a series of flattened stacked pouches called cisternae. It is located in the cytoplasm next to the endoplasmic reticulum and near the cell nucleus. The endoplasmic reticulum is a type of organelle found in eukaryotic cells that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae.

A topogenic sequence is a collective term used for a peptide sequence present at nascent proteins essential for their insertion and orienting in cellular membranes.

What is the main function of Golgi apparatus?

It has been likened to the cell's post office. A major function is the modifying, sorting and packaging of proteins for secretion. It is also involved in the transport of lipids around the cell, and the creation of lysosomes. The sacs or folds of the Golgi apparatus are called cisternae.

The endoplasmic reticulum (ER) is a type of organelle found in eukaryotic cells that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae. The membranes of the ER are continuous with the outer nuclear membrane. The endoplasmic reticulum occurs in most types of eukaryotic cells, but is absent from red blood cells and spermatozoa.

In mathematics, the Laurent series of a complex function f is a representation of that function as a power series which includes terms of negative degree. It may be used to express complex functions in cases where a Taylor series expansion cannot be applied.

Duality theory for distributive lattices

In mathematics, duality theory for distributive lattices provides three different (but closely related) representations of bounded distributive lattices via Priestley spaces, spectral spaces, and pairwise Stone spaces.

Curl theorem The curl of a vector field measures the tendency for the vector field to swirl around. Imagine that the vector field represents the velocity vectors of water in a lake.

In physics, Green's theorem finds many applications. One of which is solving two-dimensional flow integrals, stating that the sum of fluid outflows from a volume is equal to the total outflow summed about an enclosing area. In plane geometry, and in particular, area surveying, Green's theorem can be used to determine the area and centroid of plane figures solely by integrating over the perimeter.

Gravitomagnetism is a widely used term referring specifically to the kinetic effects of gravity, in analogy to the magnetic effects of moving electric charge.

effective quantum field theory

Moiré pattern Interferometry with STM Graphene applications

Moiré pattern

Application of Moire Interferometry to Strain Analysis of PCB Deformations:

Application of Moire Interferometry to Strain Analysis of PCB Deformations at Low Temperatures

Ken Wilber - Integral Operating System

Interferometry

The goal of an "integral psychology" is to honor and embrace every legitimate aspect of human consciousness under one roof. This book presents one of the first truly integrative models of consciousness, psychology, and therapy. Drawing on hundreds of sources—Eastern and Western, ancient and modern—Wilber creates a psychological model that includes waves of development, streams of development, states of consciousness, and the self, and follows the course of each from subconscious to self-conscious to superconscious. Included in the book are charts correlating over a hundred psychological and spiritual schools from around the world, including Kabbalah, Vedanta, Plotinus, Teresa of Ávila, Aurobindo, Theosophy, and modern theorists such as Jean Piaget, Erik Erikson, Jane Loevinger, Lawrence Kohlberg, Carol Gilligan, Erich Neumann, and Jean Gebser. Integral Psychology is Wilber's most ambitious psychological system to date and is already being called a landmark study in human development.

Quantum Time

Every level of interior consciousness is accompanied by a level of exterior physical complexity.

When Magic Is Seen in Twisted Graphene, That’s a Moiré

"(STM) – a technique that produces subatomic scale images of electronic motions in material surfaces at varying energies" from:

Machine learning unlocks mysteries of quantum physics

The Particle Argon is a powerful Wi-Fi development kit that you can use as a standalone Wi-Fi device or Wi-Fi enabled gateway, repeater, or endpoint for Particle Mesh networks.

https://docs.particle.io/argon/

Interior levels of consciousness and exterior levels of physical complexity with tactile sensors and wireless data transmission innovations

Quantum Gravity and Unified Theories

This division is concerned with the unification of general relativity and quantum mechanics into a theory of quantum gravity, which should also provide a consistent framework for incorporating the other fundamental forces in nature.

Kaluza–Klein theory

Triune brain

Multi Walled Carbon Nanotubes are hollow, cylindrically shaped allotropes of carbon that have a high aspect ratio (length to diameter ratio):

https://www.cheaptubes.com/product-category/multi-walled-carbon-nanotubes/

Limbic system

Despite all my rage, I'm still just a rat in a cage

Integral theory (Ken Wilber)

To coerce with out consent and Anger Turned Inward

I will have to use names of Ernie and Bert for a Python program development. A metaphor with the Golgi Aparatus and Liver and a Pack Rat/Motorcycle Mouse that may operate in such an arena and apply such concepts for software engineering in the Python programming (where these pack rat motorcycle networks may model the Golgi Aparatus and Liver as to discern a cure for muscular dystrophy)

Neocortex

"Lobo the King of Currumpaw"

Ernest Thompson Seton was an author wildlife artist, founder of the Woodcraft Indians in 1902

The Readiness of the Coyote and the Grey Wolf to respond to certain sounds and their power to produce sounds, some of which are highly musical even to us, is evidence of their having progressed far in this direction, and the enjoyment of the Pack Rat in its pile of glittering baubles is founded, as I see it on no other sense than the love of the beautiful. (Despite all my rage, I am still just a rat in a cage.)

Three Bodies Doctrine

Promoter (genetics)

In genetics, a promoter is a region of DNA that initiates transcription of a particular gene.

Human Promoters Are Intrinsically Directional

As intelligent wearables are on the rise, AI is being used to empower them into becoming true “artificial coaches” or assistants.

https://docs.particle.io/

CpG islands, Maui, Myocyte models

Learn more about CpG Island

nucleosome positioning and

histone modification states

can be used to classify promoters

associated with different types of transcription initiation patterns

CpG sites

Identification of DNA-Methylated CpG Islands Associated With Gene Silencing in the Adult Body Tissues of the Ogye Chicken Using RNA-Seq and Reduced Representation Bisulfite Sequencing

¹Genome Editing Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea
²Department of Bioinformatics, KRIBB School of Bioscience, University of Science and Technology, Daejeon, South Korea

Skeletal Muscle Tissue Engineering:
Methods to Form Skeletal Myotubes and Their Applications

Gene silencing is the regulation of gene expression in a cell to prevent the expression of a certain gene. Gene silencing can occur during either transcription or translation and is often used in research.

Electrotransfer of Peptide Nucleic Acids (PNA) in Normal and Dystrophic Muscle Tissue

Camilla Brolin, Takehiko Shiraishi, Pernille Højman, Thomas O. Krag, Peter E. Nielsen, Julie Gehl. Center for Experimental Drug and Gene Electrotransfer (C*EDGE), Department of Oncology, Copenhagen University Hospital Herlev, Herlev, Denmark; Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark; The Centre of Inflammation and Metabolism and the Centre for Physical Activity Research, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark; Neuromuscular Research Unit, Department of Neurology Rigshospitalet, University of Copenhagen, Copenhagen, Denmark

PNA is an artificial nucleic acid mimic, which has shown great promise as an antisense RNA interference agent, in particular regarding mRNA splicing modulation. However, the full potential of antisense PNA is still limited by poor cellular uptake and low in vivo bioavailability. The genetic disease Duchenne Muscular Dystrophy (DMD) has attracted particular attention for antisense drug discovery because it has been demonstrated that partial dystrophin restoration can be achieved by antisense agents causing skipping of the mutated exon.

Peptide nucleic acid

Electroporation has shown potential as a PNA delivery method in cell culture. Electroporation is already used in the clinic for treatment of cutaneous metastasis (electrochemotherapy) and the clinical field is rapidly extending to new molecules and to a range of tissues with newly developed electrodes for treatment of internal tumors. Therefore, the aim of the present project was to study the effect of electroporation upon intramuscular PNA administration. In the first study, we used a PNA targeting exon 23 in the dystrophin gene in both normal mice and in mdx mice (the animal model of DMD).

After optimizing voltage conditions, injection volume, PNA dose as well as examining different PNA modifications, we found statistically significant increase in PNA activity by 2-4 fold (determined by RT PCR) upon electrotransfer in NMRI mice. The effect lasted up to 4 weeks at the RNA level. In mdx mice lacking dystrophin protein, exon skipping was detected by immunostaining of newly synthesized dystrophin protein and by western blotting. Whole muscle transverse sections showed recovery of dystrophin at the sarcolemma in PNA treated regions in all 3 positions tested in mdx mice, but electroporation did not significantly increase the number of dystrophin positive fibers.

Clinical use of antisense therapy for treatment of DMD patients requires systemic administration of the drug because all muscles including heart muscle are affected by the disease, and must be treated. Thus further studies on systemically administered modified PNAs are needed to elucidate if electrotransfer could eventually provide therapeutic enhancement in selected muscles.

Myogenesis is the formation of muscular tissue... If placed in cell culture, most myoblasts will proliferate if enough fibroblast growth factor (FGF) or another growth factor is present in the medium surrounding the cells. When the growth factor runs out, the myoblasts cease division and undergo terminal differentiation into myotubes.

Gene drive

Selfish genetic element

No man is an island

Serenity Sells, inside sells and innovations TX HUB SBIR is partnering with Mirlok Studios in a chartered venture, suggesting:

Integral psychology may address emotional agility technology gap(s) in the lattice of developmental lines with wearable (AI) sensors.

Force Sensing Football Helmet

We are making a helmet for football or rather integrating into helmets that exist that allow us as parents to monitor impact remotely.

How Jocks and Mathletes Are Alike

Seven sports that come down to how well your neurons play.

By Sarah Zhang

Demonstrators and Product Promoters

Psychoneuroimmunology (PNI), also referred to as psychoendoneuroimmunology (PENI) or psychoneuroendocrinoimmunology (PNEI), is the study of the interaction between psychological processes and the nervous and immune systems of the human body.

When Gravity Breaks Down

Posted By Sabine Hossenfelder on Feb 15, 2019

The Electromagnetic Spectrum

Show me the Mullah: A priestly space in a vacuum Rabi oscillation:

A vacuum Rabi oscillation is a damped oscillation of an initially excited atom coupled to an electromagnetic resonator or cavity in which the atom alternately emits photon(s) into a single-mode electromagnetic cavity and reabsorbs them. Serenity Sells suggests, a microwave photon stored on-chip vacuum Rabi splitting gamma–gamma physics ( photon coupling ) interaction in a magnetic mirror manifold cubic spline. In mathematics, the pin group is a certain subgroup of the Clifford algebra associated to a quadratic space. Priestley spaces play a fundamental role in the study of distributive lattices.

The smectic phases of Liquid crystals (LCs) may be manipulated in a torus, a type of a toroid. In mathematics, a toroid is a doughnut-shaped object, a surface of revolution forming a solid body.

Serenity Sells suggests Chiral molecule packing may be computed for solutions with Spin and Pin groups as to derive an anapole magnetic torodial moment superimposed upon Soddy Circles allotropes in Quantum superposition.

In bioinformatics, clique-finding algorithms have been used to infer evolutionary trees, predict protein structures, and find closely interacting clusters of proteins.

Gravity's Rainbow is a 1973 novel by American writer Thomas Pynchon.

Rabi crops or rabi harvest are agricultural crops that are sown in winter and harvested in the spring in India.
In Judaism, a rabbi is a teacher of Torah, for priestly space sake! A random seed (or seed state, or just seed) is a number (or vector) used to initialize a pseudorandom number generator.

Molecular thumb drives: Researchers store digital images in metabolite molecules

In mathematics, the Killing form, named after Wilhelm Killing, is a symmetric bilinear form that plays a basic role in the theories of Lie groups and Lie algebras.

How long do muscle cells live?

10 to 16 years

They can last an individual's entire lifetime, but most don't. An average skeletal muscle cell's lifespan is 10 to 16 years.

Data can now be stored inside the molecules that power our metabolism

A myofiber is a multinucleated single muscle cell... Good Times Kid, Myocyte!

Myocyte

Python Programming, Myocyte modeling of COL6A1 2 3 muscle tissue engineering on CpG islands

How to Write an Effective Letter of Support

For Memorial 2019 sake and technical writing a letter of support:

Saint Ignatius is a foremost patron saint of soldiers. His feast day is celebrated on 31 July. He is the patron saint of the Basque provinces of Gipuzkoa and Biscay as well as the Society of Jesus, and was declared patron saint of all spiritual retreats by Pope Pius XI in 1922.

- Suicide rate for younger veterans increased by more than 10 percent,

Abraham Lincoln and General George B. McClellan in the general’s tent at Antietam, Maryland, October 3, 1862.

- The firearm suicide rate in Texas has increased 18% over the last decade.

Veterans

VA: Suicide rate for younger veterans increased by more than 10 percent

I survived combat in Iraq and a suicide attempt at home. But many veterans aren't so lucky.

2 Kings 4:7
"She came and told the man of God, and he said, 'Go, sell the oil and pay your debts, and you and your sons can live on the rest.’"

The Military-Petroleum Complex

America’s oil addiction has gotten it into all sorts of trouble around the world.

Proverbs 17:18
"One who lacks sense gives a pledge and puts up security in the presence of his neighbor."

Proverbs 22:7
"The rich rules over the poor, and the borrower is the slave of the lender.

With the Civil War, a new theme of death, sacrifice, and rebirth enters the new civil religion. It is symbolized in the life and death of Lincoln. Nowhere is it stated more vividly than in the Gettysburg Address, itself part of the Lincolnian “New Testament” among the civil scriptures. Robert Lowell has recently pointed out the “insistent use of birth images” in this speech explicitly devoted to “these honored dead”: “brought forth,” “conceived,” “created,” “a new birth of freedom.” Lowell goes on to say: “The Gettysburg Address is a symbolic and sacramental act. … In his words, Lincoln symbolically died, just as the Union soldiers really died—and as he himself was soon really to die. By his words, he gave the field of battle a symbolic significance that it has lacked. … He left Jefferson’s ideals of freedom and equality joined to the Christian sacrificial act of death and rebirth.”

Firearm Suicide in Texas

•Three out of every five gun deaths in Texas are suicides.

•More than half of all suicides in Texas(58%) are by firearm.

•The firearm suicide rate in Texas has increased18% over the last decade.

•84% of people who die by firearm suicide in Texas are male; and 90% are White.

US gun laws: Why it won't follow New Zealand's lead

Nehemiah 5:3-5
There were also those who said, 'We are mortgaging our fields, our vineyards, and our houses to get grain because of the famine.’ And there were those who said, “We have borrowed money for the king's tax on our fields and our vineyards. Now our flesh is as the flesh of our brothers, our children are as their children. Yet we are forcing our sons and our daughters to be slaves, and some of our daughters have already been enslaved, but it is not in our power to help it, for other men have our fields and our vineyards.”

Matthew 6:24
“No one can serve two masters, for either he will hate the one and love the other, or he will be devoted to the one and despise the other. You cannot serve God and money."

Giffords Endorses Slate of Candidates Challenging Gun Lobby-Backed Politicians

September 26, 2018

In theology, dualism can refer to the relationship between God and creation or God and the universe. This form of dualism is a belief shared in certain traditions of Christianity and Hinduism. Seamless garment philosophy, meet the Fabric of Reality. The theologian Leroy Stephens Rouner compares the dualism of Christianity with the dualism that exists in Zoroastrianism and the Samkhya tradition of Hinduism.

The Catholic Church’s grim history of ignoring priestly pedophilia – and silencing would-be whistleblowers

October 9, 2018

The excommunication of Margaret McBride occurred with the sanctioning by the American religious sister in November 2009 of an abortion at a Roman Catholic hospital in Phoenix. It was lifted in December 2011. Her decision and her subsequent excommunication aroused controversy in the areas of medical ethics and Catholic theology.

Suicide Prevention

The VA National Suicide Data Report is now available with data through 2016.

Ethicists fault bishopís action in Phoenix abortion case

Jun 8, 2010

by Tom Roberts

Deuteronomy 15:1-2
“At the end of every seven years you shall grant a release. And this is the manner of the release: every creditor shall release what he has lent to his neighbor. He shall not exact it of his neighbor, his brother, because the Lord's release has been proclaimed."

Abraham Lincoln, Civil War, Gettysburg Address, King James Bible

The Sacred Sounds of Lincoln’s Gettysburg Address

by Daniel Dreisbach

Deuteronomy 15:9
"Take care lest there be an unworthy thought in your heart and you say, ‘The seventh year, the year of release is near,’ and your eye look grudgingly on your poor brother, and you give him nothing, and he cry to the Lord against you, and you be guilty of sin."

U.S. Military Is the World's Number One Consumer of Fuel

Proverbs 3:5-6
"Trust in the Lord with all your heart, and do not lean on your own understanding. In all your ways acknowledge him, and he will make straight your paths."

Ecclesiastes 5:4
"When you vow a vow to God, do not delay paying it, for he has no pleasure in fools. Pay what you vow."

Pope Francis for first time acknowledges sexual abuse of nuns by priests

But scope of problem worldwide remains unclear as victims are reluctant to come forward and church leaders are slow to admit behavior of predator clergy.

The Case for Moderate Gun Control

Luke 7:41-50
“A certain moneylender had two debtors. One owed five hundred denarii, and the other fifty. When they could not pay, he cancelled the debt of both. Now which of them will love him more?” Simon answered, “The one, I suppose, for whom he cancelled the larger debt.” And he said to him, “You have judged rightly.” Then turning toward the woman he said to Simon, “Do you see this woman? I entered your house; you gave me no water for my feet, but she has wet my feet with her tears and wiped them with her hair. You gave me no kiss, but from the time I came in she has not ceased to kiss my feet.”

Why Are Federal Bureaucrats Buying Guns And Ammo? $158 Million Spent By Non-Military Agencies

Adam Andrzejewski

Romans 6:23
"For the wages of sin is death, but the free gift of God is eternal life in Christ Jesus our Lord."

Colossians 2:14
"By canceling the record of debt that stood against us with its legal demands. This he set aside, nailing it to the cross."

Prayer for Generosity by St. Ignatius

Romans 13:7
"Pay to all what is owed to them: taxes to whom taxes are owed, revenue to whom revenue is owed, respect to whom respect is owed, honor to whom honor is owed."

waving the banner of John 3:16, like an evangelistic football fan...

My early work is politically anarchist fiction, in that I was an anarchist for a long period of time. I'm not an anarchist any longer, because I've concluded that anarchism is an impractical ideal. Nowadays, I regard myself as a libertarian. I suppose an anarchist would say, paraphrasing what Marx said about agnostics being "frightened atheists," that libertarians are simply frightened anarchists. Having just stated the case for the opposition, I will go along and agree with them: yes, I am frightened. I'm a libertarian because I don't trust the people as much as anarchists do. I want to see government limited as much as possible; I would like to see it reduced back to where it was in Jefferson's time, or even smaller. But I would not like to see it abolished. I think the average American, if left totally free, would act exactly like Idi Amin. I don't trust the people any more than I trust the government.
- "Robert Anton Wilson: Searching For Cosmic Intelligence" - interview by Jeffrey Elliot (1980)

1 Timothy 5:8
"But if anyone does not provide for his relatives, and especially for members of his household, he has denied the faith and is worse than an unbeliever.”

Military Mothers of this earth in 2018, EZRI and the prince of peace playing with the Kumari

1 John 3:17
"But if anyone has the world's goods and sees his brother in need, yet closes his heart against him, how does God's love abide in him?"

Robert Anton Wilson on Abraham Lincoln. "A small body of determined spirits fired by an unquenchable faith in their mission can alter the course of history.".

Exodus 22:25-27
“If you lend money to any of my people with you who is poor, you shall not be like a moneylender to him, and you shall not exact interest from him. If ever you take your neighbor's cloak in pledge, you shall return it to him before the sun goes down, for that is his only covering, and it is his cloak for his body; in what else shall he sleep? And if he cries to me, I will hear, for I am compassionate."

The US Military is a Major Contributor to Global Warming

November 14, 2014 by John Lawrence

Leviticus 25:35-37
“If your brother becomes poor and cannot maintain himself with you, you shall support him as though he were a stranger and a sojourner, and he shall live with you. Take no interest from him or profit, but fear your God, that your brother may live beside you. You shall not lend him your money at interest, nor give him your food for profit."

Trump’s Racist Language of Pollution Drives His Neoliberal Fascism

Israel renames Golan Heights town "Trump Heights" in honor of U.S. president

in context of

The Trump administration is reportedly stepping up its war against climate science by forcing scientists to omit key details from a major report

Tom Porter

Deuteronomy 15:2
"And this is the manner of the release: every creditor shall release what he has lent to his neighbor. He shall not exact it of his neighbor, his brother, because the Lord's release has been proclaimed."

... left Jefferson's ideals of freedom and equality joined to the Christian sacrificial act of death and rebirth.3 3. Robert Lowell, 'On the Gettysburg Address,' Lincoln

Deuteronomy 15:6
"For the Lord your God will bless you, as he promised you, and you shall lend to many nations, but you shall not borrow, and you shall rule over many nations, but they shall not rule over you."

Why Have President Trump's Regulatory Agencies Stockpiled So Much Firepower?

Adam Andrzejewski

Why does the IRS need five million rounds of ammunition? Why did Health and Human Services purchase four million rounds over the last eight years and stockpile five submachine guns? And what about the 800,000 rounds purchased by the Social Security Administration?

Deuteronomy 23:20
"You may charge a foreigner interest, but you may not charge your brother interest, that the Lord your God may bless you in all that you undertake in the land that you are entering to take possession of it."

September 21, 2018
The Military Cost of Defending the Global Oil Supply

Proverbs 27:13
"Take a man's garment when he has put up security for a stranger, and hold it in pledge when he puts up security for an adulteress."

What You Need to Know About Trump’s $8 Billion Saudi Arms Deal

by Marcia Robiou Abrams Journalism Fellow, FRONTLINE/Columbia Journalism School Fellowship

The emperor wears no clothes, revealing:

The Pentagon’s Hidden Contribution to Climate Chang e

Gar Smith
January 18, 2016

World’s single greatest institutional consumer of fossil fuels remains exempt from reporting its pollution

Luke 16:11
"If then you have not been faithful in the unrighteous wealth, who will entrust to you the true riches?"

Pentagon Budget Includes Plan to Reduce Military Medical Force

Colossians 3:23
"Whatever you do, work heartily, as for the Lord and not for men.”

HHS SBIR/STTR PHS 2019-2 GRANT OMNIBUS/PARENT WEBINAR
May 29, 2019

Matthew Arnold was Aldous Huxley's grandfather on his mother's side for Freud's sake!

https://www.religiousfreedomcenter.org/faq/

The 1860 Oxford evolution debate took place at the Oxford University Museum in Oxford, England, on 30 June 1860, seven months after the publication of Charles Darwin's On the Origin of Species. Several prominent British scientists and philosophers participated, including Thomas Henry Huxley, Bishop Samuel Wilberforce, et al.

Matthew Arnold was an English poet and cultural critic who worked as an inspector of schools.

Adi Shankaracharya (Sanskrit: आदि शङ्कराआचार्य [aːdɪ ɕɐŋkɐɽɐ]) was an early 8th century Indian philosopher and theologian who consolidated the doctrine of Advaita Vedanta. He is credited with unifying and establishing the main currents of thought in Hinduism

Eckhart von Hochheim OP (c. 1260 – c. 1328), commonly known as Meister Eckhart or Eckehart, was a German theologian, philosopher and mystic, born near Gotha, in the Landgraviate of Thuringia (now central Germany) in the Holy Roman Empire.

The idea of corporate election expresses a Christian soteriological view that understands Christian salvation as based on "God choosing in Christ a people whom he destines to be holy and blameless in his sight". Put another way, "Election is the corporate choice of the church 'in Christ.'" Paul Marston and Roger Forster state that the "central idea in the election of the church may be seen from Ephesians 1:4": "For he [God] chose us [the Church] in him [Christ], before the creation of the world to be holy and blameless in his sight." William Klein adds:

Here [in Ephesians 1:3-4] Paul states that God chose Christians in Christ before the creation of the world to be holy and blameless in his sight. The "chosen ones" designate the corporate group to whom Paul writes with himself (and presumably all Christians) included: God chose us. The focus is not on the selection of individuals, but the group of those chosen. As Westcott notes, "He chose us (i.e. Christians as a body, v. 4) for Himself out of the world." Paul specifies the timing of this choice—it was pretemporal, before the world was created. God made the choice "in him" (that is, "in Christ"). In other words, Christ is the principal elected one, and God has chosen a corporate body to be included in him."

Song of Moses

Unconditional election (also known as unconditional grace) is a Lutheran and Reformed doctrine relating to Predestination that describes the actions and motives of God in eternity past, before He created the world, where he predestinated some people to receive salvation, the elect, and the rest he left to continue in their sins and receive the just punishment, eternal damnation, for their transgressions of God's law as outlined in the old and new Testaments of the Bible. God made these choices according to his own purposes apart from any conditions or qualities related to those persons.

The counter-view to unconditional election is conditional election, the belief that God chooses for eternal salvation those whom he foreknows will exercise their free will to respond to God's prevenient grace with faith in Christ.

For us and our country, he left Jefferson's ideals of freedom and equality joined to the Christian sacrificial act of death and rebirth.

Deuteronomy Chapter 32

Hillel the Elder (Hillel Hazaken) is one of the most influential rabbis in Jewish history. He was the head of a school, the House of Hillel, that eventually became the primary academy for Torah study prior to the destruction of the Second Temple.

Moses' seventh speech, Part 24, Laws of Usury

Psalm 15:5

Shvetaketu (IAST: Śvetaketu), also spelt Svetaketu, is a character from the Upanishadas. He was the son of sage Uddalaka, the grandson of sage Aruni, and represents the quintessential seeker of knowledge. The Upanishads entail the journey of Shvetaketu from ignorance to knowledge of the self and truth (sat).

In the Mahabharata, Shvetaketu is credited for creating the practice of the "woman being confined to one husband for life" after observing a brahmana catching his mother's hand (unintentionally) in front of his father.

The young scholar Svetaketu confronts his father Uddalaka whom conveys "That Knowledge by which we hear the unhearable,... perceive what cannon be perceived and know what cannot be known" as to 'bring fruit of a nyagrodha tree, break open the fruit to reveal the seeds, the subtle essence, conveying what you do not perceive, that is the true self":

Tat Tvam Asi (Devanagari: तत्त्वमसि, Vedic: tát túvam ási), a Sanskrit phrase, translated variously as "Thou art that," (That thou art, That art thou, You are that, or That you are, or You're it) is one of the Mahāvākyas (Grand Pronouncements) in Vedantic Sanatana Dharma. It originally occurs in the Chandogya Upanishad 6.8.7, in the dialogue between Uddalaka and his son Śvetaketu; it appears at the end of a section, and is repeated at the end of the subsequent sections as a refrain. The meaning of this saying is that the Self - in its original, pure, primordial state - is wholly or partially identifiable or identical with the Ultimate Reality that is the ground and origin of all phenomena.

Hieros gamos or Hierogamy is a sexual ritual that plays out a marriage between a god and a goddess, especially when enacted in a symbolic ritual where human participants represent the deities.

Mahāsattva

The Laṅkāvatāra Sūtra is a prominent Mahayana Buddhist sūtra. This sūtra recounts a teaching primarily between Gautama Buddha and a bodhisattva named Mahāmati, "Great Wisdom". The sūtra is set in Laṅkā, the island fortress capital of Rāvaṇa, the king of the rākṣasa demons.

Eight Consciousnesses

In 1937 Gerald Heard came to the United States, accompanied by Aldous Huxley, after having been offered the chair of historical anthropology at Duke University.

1 Corinthians 16:1

1 Timothy 5

Widows, Elders and Slaves

1 Timothy6

False Teachers and the Love of Money

Scholastic challenges as optimizing comprehension of Schubert calculus applied with a Sanchita Karma philosophy as to discern an ethical map to model such complex solutions in order to petition our human, earthly courts and government as well religious institutions, doing business as Serenity Sells:

Folkman's theorem, Ken Brown's lemma, and Solving Recurrences in Double Free set Chern Class Degrees of Freedom with Schubert calculus

Note my business partners current circumstance:

Spring Texas population 54,298 (John Donalson's current residence)

Serenity Sells proposes Force Sensing Football Helmet technology

C++ is an object-oriented programming language and has influenced other languages, such as C#, Java and the latest version of C. C++ is mostly used when the program is simple, and execution speed is the most important. ... C++ is extremely useful for AI projects

Integrating Python With Other Languages

June's Full Moon is named after the wild strawberries that start to ripen during this month.

Smart Growth in Small Towns and Rural Communities

Serenity Sells, inside sells and innovations TX HUB SBIR is partnering with Mirlok Studios in a chartered venture, suggesting:

Integral psychology may address emotional agility technology gap(s) in the lattice of developmental lines with wearable (AI) sensors.

Force Sensing Football Helmet

We are making a helmet for football or rather integrating into helmets that exist that allow us as parents to monitor impact remotely.

Smale's Problems, modulus and argument with Bridges' Transition Model

SBREFA Small Business Resource Center

For more on the SBIR technical information:

16447767

COL6A3,COL6A1,COL6A2

[Collagenopathy (Ullrich congenital muscular dystrophy, Bethlem myopathy)]

Coarse module 36- Fibroblasts genes - extracellular matrix

Spintronics (a portmanteau meaning spin transport electronics), also known as spin electronics, is the study of the intrinsic spin of the electron and its associated magnetic moment, in addition to its fundamental electronic charge, in solid-state devices. The field of spintronics concerns spin-charge coupling in metallic systems; the analogous effects in insulators fall into the field of multiferroics.
Spintronics fundamentally differs from traditional electronics in that, in addition to charge state, electron spins are exploited as a further degree of freedom, with implications in the efficiency of data storage and transfer. Spintronic systems are most often realised in dilute magnetic semiconductors (DMS) and Heusler alloys and are of particular interest in the field of quantum computing and neuromorphic computing

Introduction to Spintronics

and Spin Quantum Computation

Tribotronics refers to the combined use of tribology and electronics to control tribological systems. Tribotronic systems are designed to improve efficiency, reliability and lifetime of machine elements by active control of tribosystem inputs (such as force, torque, rotational speeds, etc.), outputs (such as motion, changes in energy, etc.) and losses (such as friction, wear, vibrations, etc.). This is in contrast to traditional tribological and mechatronic systems, where losses are seen as a function of system inputs rather than desired set-points. While the term was originally coined with a particular emphasis on the control of tribological losses, it is increasingly used to describe any active system that combines tribological and electronic components, irrespective of the process variables and set-points of the control loop.

Tribotronics

Model system of 3D engineered muscle tissue culture.

Tribotronics for Active Mechanosensation and Self‐Powered Microsystems

The Quantum Theory That Peels Away the Mystery of Measurement

A trion is a localized excitation which consists of three charged quasiparticles. A negative trion consists of two electrons and one hole and a positive trion consists of two holes and one electron. The trion itself is a quasiparticle and is somewhat similar to an exciton, which is a complex of one electron and one hole. The trion has a ground singlet state (spin s = 1/2) and an excited triplet state (s = 3/2).

PCB Piezotronics

Gustav Theodor Fechner (/ˈfɛxnər/; German: [ˈfɛçnɐ]; 19 April 1801 – 18 November 1887) was a German philosopher, physicist and experimental psychologist. An early pioneer in experimental psychology and founder of psychophysics, he inspired many 20th-century scientists and philosophers. He is also credited with demonstrating the non-linear relationship between psychological sensation and the physical intensity of a stimulus via the formula:

S=K\ln I

, which became known as the Weber–Fechner law.

In particle theory, the skyrmion (/ˈskɜːrmi.ɒn/) is a topologically stable field configuration of a certain class of non-linear sigma models. It was originally proposed as a model of the nucleon by Tony Skyrme in 1962. As a topological soliton in the pion field, it has the remarkable property of being able to model, with reasonable accuracy, multiple low-energy properties of the nucleon, simply by fixing the nucleon radius. It has since found application in solid state physics, as well as having ties to certain areas of string theory.

Quantum jump method

Psychophysics quantitatively investigates the relationship between physical stimuli and the sensations and perceptions they produce.

Physicists' finding could revolutionize information transmission

Cell force sensing using thin elastomer films

These hybrid schools of Psychophysics as well Psychoneuroimmunology have some considerations with Artificial Intelligent sensors for innovation applications.

E-textiles

Python Programming, Myocyte modeling of COL6A1 2 3 muscle tissue engineering on CpG islands

Linking phenotypes to genotypes: A newly devised gene-editing strategy

Serenity Sells suggests: Log number e, a degree of a radical argument, may be measured in myofibrils, exponential growth:

Exponential (myofibrils) growth

A myofibril (also known as a muscle fibril) is a basic rod-like unit of a muscle cell. Muscles are composed of tubular cells called myocytes, known as muscle fibers in striated muscle, and these cells in turn contain many chains of myofibrils.

Exponential Cell Growth

Trion our smart garments, tribotronic smart clothing, smart spintronic textiles, or smart fabrics for frog men from Frog Texas with quantum leaps over the logarithm L'Hospital's Rule...

In particle physics, a pion (or a pi meson, denoted with the Greek letter pi:
π
) is any of three subatomic particles:
π⁰,
π⁺, and
π⁻.

Skyrmions and Antiskyrmions in Quasi-Two-Dimensional Magnets

Each pion consists of a quark and an antiquark and is therefore a meson. Pions are the lightest mesons and, more generally, the lightest hadrons.

Magnetic materials/data storage

One particular form of skyrmions is magnetic skyrmions, found in magnetic materials that exhibit spiral magnetism due to the Dzyaloshinskii-Moriya interaction, double-exchange mechanism or competing Heisenberg exchange interactions.

Interpretations of quantum mechanics

Photonic-crystal fiber (PCF) is a class of optical fiber based on the properties of photonic crystals. It was first explored in 1996 at University of Bath, UK.

Preserving and Building Muscle Fibers

The particle-in-cell (PIC) method refers to a technique used to solve a certain class of partial differential equations. In this method, individual particles (or fluid elements) in a Lagrangian frame are tracked in continuous phase space, whereas moments of the distribution such as densities and currents are computed simultaneously on Eulerian (stationary) mesh points.

Engineered Nuclease Mediated Genetic Correction in iPSCs Derived From Duchenne Muscular Dystrophy Patient

Hongmei Lisa Li, Naoko Fujimoto, Noriko Sasakawa, Saya Shirai, Takashi Yamamoto, Knut Woltjen, Akira Watanabe, Hidetoshi Sakurai, Shinya Yamanaka, Akitsu Hotta. Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan; JSPS Research Fellow, Tokyo, Japan; Hiroshima University, Hiroshima, Japan; Hakubi Center for Advanced Research, Kyoto University, Kyoto, Japan; Yamanaka iPS Cell Special Project, JST, Kyoto, Japan; Gladstone Institute of Cardiovascular Disease, San Francisco; PRESTO, JST, Kawaguch, Japan

Duchenne Muscular Dystrophy (DMD) is the most common and severe inherited neuromuscular disease caused by the loss-of-function mutations in Dystrophin gene. Currently, no effective treatment available for DMD patients, and the large size of the Dystrophin gene hampers the delivery for gene augmentation therapy. Exon skipping to modulate mRNA splicing patterns using antisense oligonucleotide is a promising approach, however, the effect of antisense oligos is transient. Recently, targeted genome editing using engineered nucleases, such as TAL effector nucleases (TALENs) and CRISPR/Cas9, have been demonstrated to be effective and efficient to modify the target region of the genome. These promising technologies suggest the potential for the development of gene correction therapy for DMD.

In this study, we took advantages of patient-derived induced pluripotent stem cells (iPSCs) as a platform for testing various gene correction approaches to demonstrate the proof-of-concept for DMD gene thrapy. Firstly, we derived integration-free iPS cell lines from a DMD patient who lacks the exon 44 in theDystrophin gene. To correct the truncated dystrophin, we devised two approaches. One is to restore the reading frame by re-insertion of small deletion or insertion by engineered nucleases without a donor template. Another is to knock-in the deleted exon 44 with a repair template.

We constructed several TALENs and CRISPR/gRNAs to target the same site in exon 45, and demonstated high recombination efficiencies by the both systems. Isolated clones were then analized by Sanger sequencing to confirm the frame shift, and by PCR and Southern blotting to identify the knock-in clones. Since recent reports suggest the high frequencies of off-target mutagenesis by CRISPR system, we looked at the candidate off-target sites by T7 Endonuclease I assay and also examined the genome-wide protein-coding sequences by exome sequencing to assess the risk of off-target mutagenesis. So far, we obaserved no severe off-target mutagenesis introduced by both TALEN and CRISPR treatments. Finally, we differentiated the corrected DMD-iPSC clones into skeltal muscle cells in vitro by forced expression of MyoD or in vivo by injecting cells into NOD/SCID mice to form teratoma. We successfully detected the expression of dystrophin protein from two correction approaches. Our "genomic surgery" approaches using TALEN or CRISPR is effective in patient-derived iPSCs, and gene-corrected iPSCs hold a promise to serve as a cell source for cell transplantation therapies for DMD.

Growing and gaining muscles

Psychohistory is the name of a fictional science in Isaac Asimov's Foundation universe, which combined history, psychology and mathematical statistics to create a (nearly) exact science of the behavior of very large populations of people, such as the Galactic Empire.

Pluripotency

In cell biology, pluripotency (Lat. pluripotentia, "ability for many [things]") Induced pluripotent stem cells, commonly abbreviated as iPS cells or iPSCs, are a type of pluripotent stem cell artificially derived from a non-pluripotent cell, typically an adult somatic cell, by inducing a "forced" expression of certain genes and transcription factors

Induced pluripotent stem cell

Paramagnetic compounds (and atoms) are attracted to magnetic fields while diamagnetic compounds (and atoms) are repelled from magnetic fields.

In crystallography, the terms crystal system, crystal family, and lattice system each refer to one of several classes of space groups, lattices, point groups, or crystals. In crystallography, the hexagonal crystal family is one of the 6 crystal families, which includes 2 crystal systems (hexagonal and trigonal) and 2 lattice systems (hexagonal and rhombohedral). The hexagonal lattice or triangular lattice is one of the five 2D lattice types.

Transcription activator-like effector nucleases (TALEN)

A nanowire battery uses nanowires to increase the surface area of one or both of its electrodes. Some designs (silicon, germanium and transition metal oxides), variations of the lithium-ion battery have been announced, although none are commercially available. All of the concepts replace the traditional graphite anode and could improve battery performance.

Non-homologous end joining (NHEJ) is a pathway that repairs double-strand breaks in DNA

The Lennard-Jones potential (also termed the L-J potential, 6-12 potential, or 12-6 potential) is a mathematically simple model that approximates the interaction between a pair of neutral atoms or molecules. A form of this interatomic potential was first proposed in 1924 by John Lennard-Jones.

Exercising with a Muscle Disease

A localized surface plasmon (LSP) is the result of the confinement of a surface plasmon in a nanoparticle of size comparable to or smaller than the wavelength of light used to excite the plasmon. The LSP has two important effects: electric fields near the particle’s surface are greatly enhanced and the particle’s optical absorption has a maximum at the plasmon resonant frequency. The enhancement falls off quickly with distance from the surface and, for noble metal nanoparticles, the resonance occurs at visible wavelengths. For semiconductor nanoparticles, the maximum optical absorption is often in the near-infrared and mid-infrared region.

In particle physics, helicity is the projection of the spin onto the direction of momentum.

A toroidal moment is an independent term in the multipole expansion of electromagnetic fields besides magnetic and electric multipoles. In the electrostatic multipole expansion, all charge and current distributions can be expanded into a complete set of electric and magnetic multipole coefficients. However, additional terms arise in an electrodynamic multipole expansion. The coefficients of these terms are given by the toroidal multipole moments as well as time derivatives of the electric and magnetic multipole moments. While electric dipoles can be understood as separated charges and magnetic dipoles as circular currents, axial (or electric) toroidal dipoles describes toroidal charge arrangements whereas polar (or magnetic) toroidal dipole (also called anapole) correspond to the field of a solenoid bent into a torus.

The toroidal ion trap has increased ion capacity like the linear ion trap (LIT) but is still traps ions in three dimensions similar to the quadrupole ion trap (QIT).

Nonradiating anapole modes in dielectric nanoparticles

An electrochemical cell is a device capable of either generating electrical energy from chemical reactions or using electrical energy to cause chemical reactions.

Gauss's Law. The total of the electric flux out of a closed surface is equal to the charge enclosed divided by the permittivity. The electric flux through an area is defined as the electric field multiplied by the area of the surface projected in a plane perpendicular to the field.

Gauss's Law

The total of the electric flux out of a closed surface is equal to the charge enclosed divided by the permittivity.

why the perpendicular area for calculating the electric flux?

The neutron magnetic moment is the intrinsic magnetic dipole moment of the neutron, symbol μ_n. Serenity Sells suggests a Neutron Magnetic torodial dipole moment for a toroidal ion trap.

From Lattice Boltzmann Method to Lattice Boltzmann Flux Solver

Application of Buckingham Pi theorem

Diamagnetic materials are repelled by a magnetic field; an applied magnetic field creates an induced magnetic field in them in the opposite direction, causing a repulsive force. In contrast, paramagnetic and ferromagnetic materials are attracted by a magnetic field.

The flux-line lattice in superconductors

In magnetism the Stoner–Wohlfarth astroid curve is a curve that separates regions with two minima of the free energy density from those with only one energy minimum. It is a geometric representation of the Stoner–Wohlfarth model. This curve is of particular importance as discontinuous changes of the magnetization can take place when crossing it.

Phase Locking

In geometry, a simplex (plural: simplexes or simplices) is a generalization of the notion of a triangle or tetrahedron to arbitrary dimensions. In mathematics, a simplicial set is an object made up of "simplices" in a specific way. Simplicial sets are higher-dimensional generalizations of directed graphs, partially ordered sets and categories.

The Raspberry Pi 4 brings faster CPU, up to 4GB of RAM

In quantum mechanics, the Planck time (

t P

A cathode is the electrode from which a conventional current leaves a polarized electrical device. This definition can be recalled by using the mnemonic CCD for Cathode Current Departs.

In mathematics, a Casimir element (also known as a Casimir invariant or Casimir operator) is a distinguished element of the center of the universal enveloping algebra of a Lie algebra. A prototypical example is the squared angular momentum operator, which is a Casimir element of the three-dimensional rotation group.

The Schottky diode, also known as Schottky barrier diode or hot-carrier diode, is a semiconductor diode formed by the junction of a semiconductor with a metal.

"anode current into device" An anode is an electrode through which the conventional current enters into a polarized electrical device. ... The direction of conventional current (the flow of positive charges) in a circuit is opposite to the direction of electron flow...

In physics, chemistry and biology, a potential gradient is the local rate of change of the potential with respect to displacement, i.e. spatial derivative, or gradient

Field-programmable gate array

The piezoresistive effect is a change in the electrical resistivity of a semiconductor or metal when mechanical strain is applied. In contrast to the piezoelectric effect, the piezoresistive effect causes a change only in electrical resistance, not in electric potential.

The piezoelectric effect involves the interaction between the electrical and mechanical behaviour of a material. Sensor Technology Ltd.

Phase-locked loop

An inertial measurement unit (IMU) is an electronic device that measures and reports a body's specific force, angular rate, and sometimes the magnetic field surrounding the body, using a combination of accelerometers and gyroscopes, sometimes also magnetometers.

A Hail Mary pass, also known as a shot play, is a very long forward pass in American football, typically made in desperation, with only a small chance of success and/or time running out on the clock.

An atomic lattice from a 'magnetic mirror manifold' may be developed from ion flow of a 'photon positron piston'. Consider UV and IR regions of the spectrum of a silica glass refractive index of fused quartz over the spectral range from 30 nm to 1000 µm with silver surface lattice resonances. Innovative designs may be applied for a pulsed plasma thruster (PPT).

Volley theory

Energy harvesting (also known as power harvesting or energy scavenging or ambient power) is the process by which energy is derived from external sources (e.g., solar power, thermal energy, wind energy, salinity gradients, and kinetic energy, also known as ambient energy), captured, and stored for small, wireless autonomous devices, like those used in wearable electronics and wireless sensor networks.

Permittivity

Fluorescence refers to the physical property of an object absorbing light at one wavelength and then reemitting it at another wavelength. If a molecule absorbs the light of one wavelength and emits it in another (i.e., fluoresces), we call that molecule a fluorophore.

The relative permittivity of a material is its permittivity expressed as a ratio relative to the vacuum permittivity. (including for AI wearable applications)

Dielectric Constants at 20°C

A crystal oscillator is an electronic oscillator circuit that uses the mechanical resonance of a vibrating crystal of piezoelectric material to create an electrical signal with a precise frequency.

Lyotropic liquid-crystalline phases are abundant in living systems but can also be found in the mineral world. For example, many proteins and cell membranes are liquid crystals... On heating a particular type of LC molecule (called mesogen) may exhibit various smectic phases followed by the nematic phase and finally the isotropic phase as temperature is increased.

A pulsed plasma thruster (PPT), also known as a plasma jet engine, is a form of electric spacecraft propulsion.

wear one's heart on one's sleeve

and have one's heart on one's sleeve. Fig. to display one's feelings openly and habitually, rather than keep them private.

Let’s Talk About George H.W. Bush’s Role in the Iran-Contra Scandal

The Anglo-Persian Oil Company (APOC) was a British company founded in 1908 following the discovery of a large oil field in Masjed Soleiman, Iran.

BP’s Long History Of Destroying The World

CIA admits role in 1953 Iranian coup

Arbusto and James Bath
A Mysterious Mover of Money and Planes
By Jonathan Beaty

BP and Iran: The Forgotten History

How George H.W. Bush’s Pardons for Iran-Contra Conspirators Set the Stage for Trump’s Impunity

Operation Earnest Will (24 July 1987 – 26 September 1988) was the American military protection of Kuwaiti-owned tankers from Iranian attacks in 1987 and 1988, three years into the Tanker War phase of the Iran–Iraq War. It was the largest naval convoy operation since World War II.

Trump controlled by Mossad

By Timothy Fitzpatrick
October 29, 2016

Trump’s ties to Mossad/CIA Pedophile Ring

Gordon Duff, Senior Editor
- January 28, 2018

Ex-Israeli Premier Plays Down Relationship With Epstein

By The Associated Press

July 11, 2019

'TRUMP DONATED TO PEDOPHILE GROUP'; SEX SLAVES

Deflecting blame, Acosta pointed finger at others. Why they may have some explaining to do

Neil Bush Pedophile Network

Emperors Club: All About Eliot Spitzer’s Alleged Prostitution Ring

By Katherine Thomson

Out of Prison, Ex-Procurers for Client 9 Get New Jobs

By WILLIAM K. RASHBAUMSEPT. 12, 2011

Why did the United States go to war in Iraq in 2003?

A look at the rationales for going to war in Iraq, from WMD and terrorism to democracy in the Middle East. Americans were told by President Bush and his administration that the U.S. was going to war with Iraq because of the imminent threat of Saddam's weapons of mass destruction and ties to terrorism.

From 1981-1986 the chief financial officer of Arbusto Energy was Mike Conaway. Conaway currently is the U.S. Representative for Texas's 11th congressional district. Notice Texas 11th congressional district, March 4, 1883 – January 3, 2005 was a Democratic Party, until CFO of Arbustor Energy, Mike Conaway a Republican business associate of George Bush, was ushered into a Republican Party run, Texas's 11th congressional district for the first time in the history of the district.

Application of Moire Interferometry to Strain Analysis of PCB Deformations at Low Temperatures

Trion, Plasmon, Phonon...

Military Soft Body Armor — Protecting the protectors.

Piezotronics

How 3-D Printed Body Armor Is Useful for Both American Forces and Terrorists

Spintronics

Interceptor Body Armor

Tribotronics

Tribotronic bipolar junction transistor for mechanical frequency monitoring and use as touch switch

Serenity Sells SBIR

SBIR Phase II Funding Opportunity – Seeking Methods to Improve Reproducibility of Human iPSC Derivation, Growth and Differentiation

The National Institute of General Medical Sciences (NIGMS) is seeking Small Business Innovation Research (SBIR) Phase II applications to develop methods that improve the reproducibility of human induced pluripotent stem cell (iPSC) derivation, growth, and differentiation (RFA-GM-19-001).

As you may know, realizing the full potential of human iPSCs for both research and clinical practice has been limited by the significant variability in reprogramming efficiency, differentiation potential, and cell growth and stability. This Funding Opportunity Announcement (FOA) will support SBIR projects to develop novel, reliable and cost-effective methods to standardize and increase the utility and reproducibility of iPSCs at all stages, from their derivation to their research and clinical applications.

Human induced pluripotent stem cells (iPSCs) have been used with great success to mimic the differentiation of a variety of tissues, understand early development and study human diseases. Despite approaches that have made the derivation, growth and differentiation of iPSCs more efficient, there remains significant variability in reprogramming efficacy, genomic integrity and developmental potential of iPSCs derived from a single fibroblast or tissue sample. Thus, iPSCs derived from the same sample may differ in their in vitro growth characteristics and their ability to re-differentiate into the desired tissue type. A variety of issues may affect derivation of the iPSCs and their growth, stability and differentiation, including the specific characteristics of the starting cell or tissue sample (e.g., age of donor, tissue type and anatomical location, physiological and disease state), the methods and protocols used to induce pluripotency (e.g., transcription factors, small molecules, cell fusion), the choice of growth factors and other culture conditions, method of storage of cell lines, etc. Further challenges include growing and maintaining sufficient quantities of iPSC lines in culture without changes in their properties, as well as the ability of multiple investigators to identify and authenticate iPSC lines as part of their research.

Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from adult cells.

Congressional Directed Medical Research Programs

The EBSA Employee Benefits Security Administration is an agency of the United States Department of Labor responsible for administering, regulating and enforcing the provisions of Title I of the Employee Retirement Income Security Act of 1974.

Researchers

EBSA's research expands knowledge of employee benefits and their role in meeting the economic security needs of the Nation. EBSA conducts and disseminates economic and policy research designed to advance EBSA's mission, "improving health benefits and retirement security for all workers." EBSA's research program fulfills the Secretary of Labor authorization and directive to conduct research related to employee benefit plans under Section 513(a) of ERISA.